Alkaline Phosphatase #CAS9001-78-9
Alkaline Phosphatase #CAS9001-78-9
The Alkaline Phosphatase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity.
Alkaline Phosphatase is Dimeric protein having a molecular weight of 140 kDa, one Zn++ ion is tightly bound to each subunit, and another less tightly bound is involved in the catalytic reaction. Mg++ stimulates the catalysis. The binding site for Mg++ is different to that of Zn++, but will be occupied by excess Zn++ followed by loss of enzyme activity.
Application of Alkaline Phosphatase
Alkaline Phosphatase, calf intestine, EIA GradeDephosphorylation of cloning vector DNA to prevent recircularization during ligation, dephosphorylation of DNA prior to end-labeling using T4 Polynucleotide Kinase, treatment of dNTPs in PCR reactions prior to sequencing or SNP analysis, dephosphorylation of DNA and RNA. It is also used to remove the 5-terminal phosphate from nucleic acids during molecular cloning reactions.
Hydrolyzes 5′-terminal monophosphate (dephosphorylation) from DNA and RNA. Prevents fragments from self annealing. 5′-nucleic acid targeting for probes.
Alkaline Phosphatase is commonly used to remove the 5′-terminal phosphate from nucleic acids.
| Alkaline Phosphatase Chemical Properties |
| density | 1.12 g/mL at 20 °C |
| storage temp. | -20°C |
| form | suspension |
| color | white |
| Specific Gravity | 1 |
| PH | ~7.6 |
| biological source | mouse |
| Water Solubility | It is soluble in water. |
| Specific Activity | 30-90units/mg protein (modified Warburg-Christian, in glycine buffer) |
| Stability: | Stability Incompatible with strong oxidizing agents |
| Cosmetics Ingredients Functions | SKIN CONDITIONING |
| InChIKey | ITZMJCSORYKOSI-XUAWLZQMNA-N |
| SMILES | C(O)(=O)[C@@H](NC([C@@H]1CCCN1C(CNC([C@@H]1CCCN1C(=O)[C@@H](N)C)=O)=O)=O)CCC/N=C(\N)/N |&1:3,6,15,22,r| |
| EPA Substance Registry System | Alkaline phosphatase (9001-78-9) |
| Absorption | 7.6 at 278nm (10 mg AP/ml) |
| Safety Information |
| Hazard Codes | B,Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26-36-24/25-22-23 |
| WGK Germany | 3 |
| F | 3-10 |
| TSCA | TSCA listed |
| HS Code | 35079090 |
| Storage Class | 11 - Combustible Solids |
| Hazard Classifications | Resp. Sens. 1 |
| Toxicity | An enzyme with an alkaline pH optimum that hydrolyzes phosphate monoesters. Elevation of its activity in the serum usually indicates obstructive jaundice, Paget’s disease (osteitis deformans), or bone carcinoma. |
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