Phenazine methosulfate #CAS299-11-6
Phenazine methosulfate #CAS299-11-6
Phenazine is a free radical generator. It has been used as an electron transfer reactant in cell viability assays. Phenazine (10 μM) induces ssDNA break formation in the presence of the reducing agent NADPH in a cell-free plasmid cleavage assay when used at a concentration of 10 μM. It induces oxidative DNA damage in an alkaline comet assay and apoptosis in A375 melanoma cells when used at a concentration of 10 μM. Phenazine (20 nM) oxidizes cysteine-containing proteins in HepG2 cells.Phenazines are heterocyclic compounds produced as secondary metabolites by bacteria.
| Phenazine methosulfate Chemical Properties |
| Melting point | 158-160 °C (dec.)(lit.) |
| density | 1.3395 (rough estimate) |
| refractive index | 1.6930 (estimate) |
| storage temp. | -20°C |
| solubility | H2O: 0.2 g/mL at 20 °C, clear, deep orange |
| form | Crystalline Powder |
| color | Dark yellow to brown or khaki |
| Water Solubility | soluble in water (approximately 200 mg/ml). |
| Sensitive | Light Sensitive |
| Merck | 14,6109 |
| BRN | 3898869 |
| Stability: | Stable. Incompatible with strong oxidizing agents. |
| InChI | InChI=1S/C13H11N2.CH4O4S/c1-15-12-8-4-2-6-10(12)14-11-7-3-5-9-13(11)15;1-5-6(2,3)4/h2-9H,1H3;1H3,(H,2,3,4)/q+1;/p-1 |
| InChIKey | RXGJTUSBYWCRBK-UHFFFAOYSA-M |
| SMILES | C12C=CC=CC1=NC1=CC=CC=C1[N+]=2C.S([O-])(=O)(=O)OC |
| CAS DataBase Reference | 299-11-6(CAS DataBase Reference) |
| EPA Substance Registry System | Phenazinium, 5-methyl-, methyl sulfate (299-11-6) |
| Safety Information |
| Hazard Codes | Xi,Xn,T |
| Risk Statements | 36/37/38-68-46 |
| Safety Statements | 26-36/37/39-53-45 |
| RIDADR | 2811 |
| WGK Germany | 3 |
| RTECS | SG1645000 |
| F | 8 |
| TSCA | TSCA listed |
| HS Code | 29339900 |
| Storage Class | 11 - Combustible Solids |
Application of Phenazine methosulfate
Phenazine Methosulfate is used in tyrosine transaminase test and for enzymic determination of ethanol in blood by the colorimetric micromethod. Used with ascorbic acid to determine nitric oxide reductase activity. Since the reduced PMS is easily oxidized by oxygen, it is used in assays as an electron carrier between enzymes and oxygen, cytochrome c, indophenols, or tetrazolium salts. The reduced PMS is used as an electron donor to reduce cytochrome c or in photosynthetic experiments. Usage of PMS for detection of specific dehydrogenases has been reported.
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